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A multiplex PCR assay able to simultaneously detect Torque teno virus isolates from phylogenetic groups 1 to 5 BJMBR
Devalle,S.; Niel,C..
Torque teno virus (TTV) is a circular, single-stranded DNA virus that chronically infects healthy individuals of all ages worldwide. TTV has an extreme genetic heterogeneity which is reflected in its current classification into five main phylogenetic groups (1-5). Using specific PCR assays, it has been shown that many individuals are co-infected with TTV isolates belonging to different phylogenetic groups. Here, a multiplex PCR assay was developed, using five recombinant plasmids. Each plasmid carried an insert of different size issued from a TTV isolate belonging to a different group. The assay was able to simultaneously amplify DNAs of TTV isolates belonging to all five phylogenetic groups. Multiplex PCR was then tested satisfactorily on DNAs extracted...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Brazil; Co-infections; Multiplex; PCR; Torque teno virus.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000600006
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Research of Torque teno virus (TTV) in Serum and Total Blood of Brazilian Non-Human Primates and in Chicken Plasma (Gallus gallus domesticus) by the PCR N22 Region International Journal of Morphology
Catroxo,M. H. B; Nishiya,A; Sabino,E; Teixeira,P. S; Petrella,S; Milanelo,L; Vieira,J.C.F; Diaz,R. S.
Torque teno virus (TTV) is a recently discovered DNA virus that was originally isolated from a Japanese patient (initials, TT) with post-transfusion hepatitis of unknown aetiology. TTV is an circular DNA virus classified recently together with related Torque teño minivirus, into a new genus called Anellovirus. Infection TTV has been detected in a range of non-human primates as well as domestic animals. The purpose of this study was to search TTV in the serum and total blood of Brazilian monkeys and in plasma of domestic chickens by seminested PCR of coding region (N22), followed by a genomic sequence and phylogenetic analysis. No serum sample was amplified. TTV DNA was detected in total blood from 3 (4%) out of 75 brown-capuchin (Cebus apella) and from 1...
Tipo: Journal article Palavras-chave: Torque teno virus; Non-humans primates; Coding region; Amino acid sequencing.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022008000200020
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Torque teno virus among dialysis and renal-transplant patients BJM
Takemoto,Angélica Yukari; Okubo,Patrícia; Saito,Patricia Keiko; Yamakawa,Roger Haruki; Watanabe,Maria Angélica Ehara; Veríssimo da Silva Junior,Waldir; Borelli,Sueli Donizete; Bedendo,João.
Patients who undergo dialysis treatment or a renal transplant have a high risk of blood-borne viral infections, including the Torque teno virus (TTV). This study identified the presence of TTV and its genome groups in blood samples from 118 patients in dialysis and 50 renal-transplant recipients. The research was conducted in a hospital in the city of Maringá, state of Paraná. The viral DNA, obtained from whole blood, was identified by using two nested Polymerase Chain Reactions (PCR). The frequencies of TTV were 17% and 36% in dialysis patients using the methodology proposed by Nishizawa et al. (1997) and Devalle and Niel (2004), respectively, and 10% and 54% among renal-transplant patients. There was no statistically significant association between the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Torque teno virus; Renal dialysis; Kidney transplant; Infection.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100307
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